Potential of Magnetic Hyperthermia to Stimulate Localized Immune Activation

Magnetic hyperthermia (MH) harnesses the heat-releasing properties of superparamagnetic iron oxide nanoparticles (SPIONs) and has potential to stimulate immune activation in tumor microenvironment whilst sparing surrounding normal tissues. To assess feasibility localized MH vivo, SPIONs are injected intratumorally their fate tracked by Zirconium-89-positron emission tomography, histological analysis, electron microscopy. Experiments show that an average 49% (21–87%, n = 9) retained within or immediately tissue. In situ heating is subsequently generated exposure externally applied alternating magnetic field monitored thermal imaging. Tissue response hyperthermia, measured immunohistochemical image reveals specific heat-shock protein expression following treatment. Tumor growth inhibition also observed. evaluate effects on landscape, flow cytometry used characterize cells from excised tumors draining lymph nodes. Results influx activated cytotoxic T cells, alongside increase proliferating regulatory Complementary changes found conclusion, results indicate biologically reactive achievable vivo can generate consistent with anti-tumor response.